MabSelect™ VL protein L minicolumns with a redesigned protein L ligand. Capture antibody Fabs, bispecifics, and other variants containing a kappa light chain with high binding capacity and improved alkaline stability. Supports high-throughput chromatography process development (HTPD) and scale-up by allowing parallel screening of chromatographic conditions using a minicolumn format and robotic workstation. High-throughput workflow allows investigation of enlarged experimental workspace for better process understanding. Prefilled with MabSelect™ VL resin with high dynamic binding capacity for efficient capture of target molecules provides good resolution for product-related impurities in the capture of bispecific antibodies. Built for a diversifying pipeline As the diversity of antibody variants increases, so does the need for chromatography resins that can capture and purify these variants. As an alternative to protein A, protein L resin can be used to capture antibody Fabs, bispecific antibodies, and other molecules containing kappa variants of the variable light (VL) chain. Protein L binds human kappa light chain subtypes 1, 3, and 4 and mouse kappa light chain subtype 1. Redesigned protein L ligand MabSelect™ VL protein L resin showed a two-fold increase in binding capacity compared to our Capto™ L chromatography resin. This higher capacity provides productivity advantages in research and biomanufacturing. The MabSelect™ VL protein L resin can withstand cleaning with 0.1 M NaOH, thanks to multimerization and modification of one of the domains of the native protein L ligand. MabSelect™ VL protein L resin is also available in prepacked HiTrap™ columns for research applications and as bulk resin in different pack sizes. Contact your Cytiva sales representative for more information. *RoboColumn® is a trademark of Repligen GmbH.