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Catalog No. CYT17505401
Part No. 17505401
Supplier Cytiva
HiTrap SP FF
Net Price
213
Availability

4 weeks

PROPERTIES
  • Bed volume
    1
  • Flow rate
    < 4 ml/min
  • Pressure max. (over the packed bed during operation)
    5 bar [0.5 MPa] (70 psi)
  • Pack size
    5 × 1 mL
  • Bed height
    25 mm
  • Column i.d.
    7
  • Application
    For method scouting, group separations, sample concentration and sample clean-up of charged biomolecules
  • Storage
    4 to 30°C, 0.2 M Sodium Acetate in 20% Ethanol
  • Bed dimensions
    7 × 25 mm
DESCRIPTION
HiTrap SP FF is prepacked with SP Sepharose Fast Flow, and is a strong cation exchanger for small-scale protein purifications as well as screening of binding and elutions conditions. Convenient and affordable for fast, easy ion exchange separations either alone or connected in series. The industry standard for ion exchange chromatography. High flow rates and good scale-up potential. Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient. Predictable scale-up Q, SP, DEAE, and CM Sepharose Fast Flow are based on a robust, 6% highly cross-linked beaded agarose matrix with good flow properties and high loading capacities. ANX Sepharose 4 Fast Flow (high sub) is based on 4% highly cross-linked beaded agarose. This results in a resin with higher porosity, which is particularly useful for the purification of high molecular mass proteins.
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