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Catalog No. CYT17511001
Part No. 17511001
Supplier Cytiva
HiTrap IgM Purification HP
Net Price
945
Availability

4 weeks

PROPERTIES
  • Chromatography technique
    Antibody affinity chromatography
  • Bed volume
    1
  • Flow rate
    < 4 ml/min
  • Pressure max. (over the packed bed during operation)
    5 bar [0.5 MPa] (70 psi)
  • Pack size
    5 × 1 mL
  • Bed height
    25 mm
  • Column i.d.
    7
  • Application
    Used for purification of IgM
  • Storage
    4 to 8°C, 20% Ethanol
  • Bed dimensions
    7 × 25 mm
DESCRIPTION
HiTrap IgM Purification HP is a 1 mL column prepacked with Sepharose High Performance for fast and efficient purification of monoclonal IgM. Optimized for fast, efficient purification of monoclonal IgM from hybridoma cell culture. Prepacked with a thiophilic adsorption resin, 2-mercaptopyridine coupled to Sepharose High Performance. Recovery of IgM is high with excellent activity retention. The top and bottom frits are manufactured from porous polyethylene Columns are delivered with a stopper on the inlet and a snap-off end on the outle HiTrap IgM Purification HP is prepacked with a thiophilic adsorption resin, 2-mercaptopyridine coupled to Sepharose High Performance. Thiophilic adsorption is promoted by water-structuring salts. It has been suggested that the interaction of IgM and ligand results from combined electron donating and accepting action of the ligand or, alternatively, a mixed mode hydrophilic-hydrophobic interaction.
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