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Catalog No. CYT17505501
Part No. 17505501
Supplier Cytiva
HiTrap DEAE FF
Net Price
213
Availability

4 weeks

PROPERTIES
  • Bed volume
    1
  • Flow rate
    < 4 ml/min
  • Pressure max. (over the packed bed during operation)
    5 bar [0.5 MPa] (70 psi)
  • Pack size
    5 × 1 mL
  • Bed height
    25 mm
  • Column i.d.
    7
  • Application
    For method scouting, group separations, sample concentration and sample clean-up of charged biomolecules
  • Storage
    4 to 30°C, 20% Ethanol
  • Bed dimensions
    7 × 25 mm
DESCRIPTION
HiTrap DEAE FF is prepacked with DEAE Sepharose Fast Flow, and is a weak anion exchanger for small-scale protein purifications as well as screening of binding and elutions conditions. Convenient and affordable for fast, easy ion exchange separations either alone or connected in series. The industry standard for ion exchange chromatography. High flow rates and good scale-up potential. Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient. Predictable scale-up Q, SP, DEAE, and CM Sepharose Fast Flow are based on a robust, 6% highly cross-linked beaded agarose matrix with good flow properties and high loading capacities. ANX Sepharose 4 Fast Flow (high sub) is based on 4% highly cross-linked beaded agarose. This results in a resin with higher porosity, which is particularly useful for the purification of high molecular mass proteins. The active end of the charged group is the same for DEAE Sepharose Fast Flow and ANX Sepharose Fast Flow (high sub), the difference is the length of the carbon chain of the charged group. DEAE Sepharose Fast Flow has a diethylaminoethyl-group bound to the agarose whilst ANX Sepharose 4 Fast Flow (high sub) has a diethylaminopropyl group attached.
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