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Catalog No. CYT17544122
Part No. 17544122
Supplier Cytiva
HiTrap Capto S, 5
Net Price
235.5
Availability

4 weeks

PROPERTIES
  • Chromatography technique
    Ion Exchange Chromatography
  • Bed volume
    1 mL
  • Flow rate
    < 4 ml/min
  • Pressure max. (over the packed bed during operation)
    5 bar [0.5 MPa] (70 psi)
  • Pack size
    5 × 1 mL
  • Bed height
    25 mm
  • Column i.d.
    7
  • Storage
    4 to 30°C, 0.2 M Sodium Acetate in 20% Ethanol
  • Bed dimensions
    7 × 25 mm
DESCRIPTION
HiTrap Capto S chromatography column is prepacked with a strong cation exchange modern resin for small scale protein purification. Suitable for screening of selectivity, binding and elution conditions, and small-scale purifications. Sulfoethyl (S) strong cation exchanger. Optimized for capture and intermediate protein purification. Convenient HiTrap format for easy connection to a syringe, peristaltic pump, or chromatography system such as an ÄKTA system. Protein capture and intermediate purificationCapto S resin couples a sulfoethyl strong cation exchanger to a chemically modified, high-flow agarose matrix. The rigid agarose matrix provides greater particle rigidity without compromising pore size, which allows for high bed volume and purification of viscous samples at high flow rates. High dynamic binding capacity is achieved with a dextran surface extender that coats the agarose matrix. The resin’s high binding capacity, high flow rate, and low back pressure reduce process cycle times and improve productivity at process scale.Successful use in published researchApplications for these strong cation exchange chromatography columns include: Analysis of the binding properties of recombinant Plasmodium falciparum VAR2CSAPurification of a new actinoporin Gixantoxin-4Purification and characterization of the staphylococcal bacteriophage lysin CHAP(K)Other Capto S resin formatsCapto S resin is also available as bulk resin and in other prepacked formats. Therefore, it is possible to use the same resin for development work, pilot studies, and routine production.Other Cytiva ion exchange productsCytiva's solutions include a variety of ion exchange chromatography resins, such as Capto, Sepharose, and SOURCE.
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