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Catalog No. CYT17514401
Part No. 17514401
Supplier Cytiva
HiTrap Benzamidine FF (High Sub), 1 x 5ml
Net Price
561
Availability

4 weeks

PROPERTIES
  • Chromatography technique
    Group specific affinity
  • Bed volume
    1
  • Flow rate
    < 4 ml/min
  • Pressure max. (over the packed bed during operation)
    5 bar [0.5 MPa] (70 psi)
  • Pack size
    5 × 1 mL
  • Bed height
    25 mm
  • Column i.d.
    7
  • Application
    For the removal and/or purification of serine proteases e.g. thrombin
  • Storage
    2 to 8°C, 0.05 M Sodium Acetate, pH 4.0 in 20% Ethanol
  • Bed dimensions
    7 × 25 mm
DESCRIPTION
HiTrap Benzamidine FF (high sub) are prepacked with Benzamidine Sepharose 4 Fast Flow (high sub) and designed specifically for the removal and/or purification of serine proteases such as thrombin. Effective removal of thrombin and factor Xa after tag cleavage of recombinant proteins. Removal of serine proteases is easily done directly from serum, monoclonal cell supernatants and bacterial lysates. HiTrap Benzamidine FF for convenient, one-step removal and/or purification of trypsin, trypsin-like serine proteases, and zymogens including urokinase and prekallikrein. High binding capacity. The columns can be operated using syringe, peristaltic pump or liquid chromatography system such as ÄKTA design or FPLC System. HiTrap Benzamidine FF (high sub) are prepacked 1 mL and 5 mL columns for convenient, one-step removal and/or purification of trypsin, trypsin-like serine proteases, and zymogens including urokinase and prekallikrein. Removal of serine proteases is easily done directly from serum, monoclonal cell supernatants and bacterial lysates. The medium, Benzamidine Sepharose 4 Fast Flow (high sub), is also available as lab packages and is an excellent choice for scale-up. The columns can be operated using syringe, peristaltic pump or liquid chromatography system such as ÄKTAdesign or FPLC System.
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