DESCRIPTION
HisTrap FF Crude columns are high binding capacity IMAC columns for his-tagged protein purification when scalability is needed.
High binding capacity – binds up to 40 mg his-tag protein/mL resin.
Excellent flow properties – great performance even at high flow rates.
Optimized for unclarified samples – short purification time can minimize potential negative effects, such as degradation and oxidation of proteins.
HiTrap column format – compatible with syringe, pump, or chromatography system such as ÄKTA systems or other FPLC systems.
His-tag protein purification from unclarified lysates
HisTrap FF crude is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow. After thorough cell disruption, it is possible to load the unclarified lysate on the column, without precentrifugation and filtration.
If you plan to load unclarified samples, it is important to ensure that lysis is as complete as possible. We recommend adding lysozyme and DNase I, followed by mechanical lysis (e.g., by sonication) for a longer duration than typically performed.
Tips and tricks for using these IMAC columns
Interested in maximizing the performance of your column? Watch Cytiva's HisTrap FF crude column video to get hints and recommendations.
Successful use in published research
These columns are used in a variety of applications, including:
Purification of a his-tagged ferritin protein from unclarified E. coli lysates
Purification of unphosphorylated human ribosomal protein
Recombinant protein purification to study the mechanism of action of antibiotic NXL101
Other Cytiva products for purifying his-tagged proteins
Cytiva provides a large variety of Life Sciences solutions for IMAC protein purification. For routine his-tagged protein purification or when high resolution is a priority, consider HisTrap HP columns.
Not sure which product to use? Let Cytiva help you find the right IMAC purification resin or column for your needs for your needs.
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