HiScale™ empty chromatography columns are a family of pressure-stable, easy to pack, columns for medium to high pressure liquid chromatography using a chromatography system. Easy to pack: convenient column measurement scale, nonrotating plunger mechanism, and ergonomically designed components for easier adjustment during column packing Pressure-stable: built to withstand pressures up to 20 bar (2 MPa, 290 psi) for compatibility with modern BioProcess™ resins Versatile: dual adapters and axial compression capability allow for a large range of bed heights and column packing protocols Variety of column dimensions: choose from 10, 16, 26, or 50 mm inner diameter, and 200 mm or 400 mm length to accommodate bed volumes up to 785 mL Scalable: the HiScale™ column range is designed to facilitate robust process scaleup, as well as cost-efficient and accurate scaledown for applications such as viral clearance studies High flexibility: equipped with two adapters allowing for a large range of bed heights. What comes in each HiScale™ column chromatography packages? Each HiScale™ column is delivered with a column tube, outer protection tube, two adapters, PEEK tubing, and unions at both ends for easy connection to valves, pumps, and monitors. For HiScale™ 10/40, connectors are provided in the accessory kit included in the package. HiScale™ column design for medium to high pressure liquid chromatography HiScale™ pressure‐stable, empty columns are designed for process development and preparative chromatography using ÄKTA protein purification systems. These chromatography columns withstand pressures up to 20 bar, with a user‐friendly design that supports process optimization through simplified and reproducible operations. The QuickLock mechanism of the adapter shaft facilitates rapid and easy movement of the adapter, simplifying adjustments as well as disassembly and cleaning. The entire shaft may be rinsed after column packing, minimizing the risk of bacterial growth. The column’s measurement scale helps users to predict and reach a predefined bed height. HiScale™ columns are based on the well‐established line of XK columns. They are compatible with all methods currently applicable to XK columns. Viral clearance and other scaledown studies with HiScale™ 10/40 HiScale™ 10/40 columns are especially suitable for scaledown studies used in, for example, viral clearance evaluation in downstream processing. The small inner diameter and flexible adapters minimize costly sample and viral spike volumes. The robust design of HiScale™ 10/40 allows for controlled bed heights and gives reproducible results offering an effective scaled down model of the large-scale downstream process. Resin compatibility for medium to high pressure liquid chromatography HiScale™ chromatography columns are compatible with all Cytiva resins. For columns packed with SOURCE 15 and SOURCE 30, a 10 µm net ring should be used. ÄKTA system and other chromatography system compatibility The user-friendly design of the HiScale™ column facilitates trouble-free operation, with fittings for direct connection to ÄKTA systems. These empty chromatography columns are also compatible with other high performance liquid chromatography systems that have the appropriate pressure capacity. Successful use in published research See these examples of successful applications of HiScale™ columns in the scientific literature: scaledown studies for commercial biotherapeutics manufacturing capture of single-chain fragment variable (ScFv) antibodies using Capto L affinity resin purification of sialylated glycans using Q Sepharose Fast Flow anion exchange resin desalting of a potential malaria vaccine sample using Sephadex G-25 resin before multimodal chromatography HiScale™ column packing Watch our comprehensive tutorial on how to pack a HiScale™ column.