DESCRIPTION
HiPrep 16/10 Phenyl HP is a prepacked column designed for preparative hydrophobic interaction chromatography (HIC) with good resolution.
Aromatic HIC resin for intermediate purification and polishing.
Selectivity similar to Phenyl Sepharose 6 Fast Flow (Low Sub) but higher resolution due to the smaller 34 µm bead size.
Provides fast, easy high-capacity separations in a convenient format.
Phenyl Sepharose HP chromatography resin is based on a matrix of highly cross-linked agarose beads, which have been derivatized with phenyl groups. These are attached to the agarose matrix via uncharged, chemically stable ether linkages. The result is a truly hydrophobic resin with no ionic properties.
High mechanical strength of the matrix allows for high flow rates, together with good resolution. Capacities vary according to the protein applied and its concentration. Loading capacity will also vary depending on the salt concentration, pH, and temperature at which the protein is applied.
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