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Catalog No. MN760741.20
Part No. 760741.20
Supplier MACHEREY‑NAGEL
EC HPLC column (analytical), NUCLEODUR 100-3 NH2-RP, 3 µm, 125x2 mm
Net Price
Special offer
Availability

6 weeks

PROPERTIES
  • Recommended application(s)
    HPLC, Hydrocarbons under NP conditions, Polar compounds under RP conditions (sugars, DNA bases)
  • Storage temperature
    RT
  • Scope of delivery
    1x analytical HPLC column, instruction leaflet, certificate of analysis
  • Hazardous material
    No
  • Method
    HPLC, analytical
  • Brand
    NUCLEODUR
  • Base material
    NUCLEODUR - high purity silica
  • Phase
    NUCLEODUR 100-3 NH2-RP
  • USP listing
    L8
  • Surface chemistry
    Aminopropyl (NH2) modification
  • Mode
    Multi-mode (NP and RP), Reversed phase (RP)
  • Type
    EC HPLC column (analytical)
  • Hardware
    Stainless steel
  • Column length
    125 mm
  • Column inner diameter
    2 mm
  • Particle type
    Fully porous particles (FPP)
  • Particle shape
    Spherical
  • Particle size
    3 µm
  • Eluent in HPLC column
    Acetonitrile – water
  • Endcapped
    No
  • Carbon content
    2.5 %
  • pH stability
    2.0–8.0
  • Pore size
    110 Å
  • Specific surface according to BET
    340 m²/g
  • Temperature stability (max)
    60 °C
  • Pressure stability (max)
    600 bar
  • Column volume
    0.37 mL
DESCRIPTION
This HPLC column is packed with polar aminopropyl modification, based on high purity NUCLEODUR silica gel. This HPLC phase is able to generate a clearly recognizable different retention behavior compared to purely alkyl-functionalized surface modifications. Besides cyano modifications, amino modifications belong to the most frequently used polar silica phases. Amino phases can be run in the RP mode using aqueous-organic eluent mixtures as well as in the NP mode, e.g., with hexane as mobile phase. Also anion exchange chromatography of anions and organic acids using common buffers (e.g., acetate or phosphate) in conjunction with organic modifiers (e.g., acetonitrile) is possible. Separation mechanism based on polar interactions, hydrophobic interactions and weak ion exchange interactions.
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