Aldehydes, Basic, neutral or acidic drugs, Derivatized amino acids, Fat-soluble vitamins, Ketones, Pesticides, Phenolic compounds, Reversed phase, RP applications HPLC
Storage temperature
RT
Scope of delivery
1x analytical HPLC column, instruction leaflet, certificate of analysis
Hazardous material
No
Method
HPLC, analytical
Brand
NUCLEOSIL
Base material
NUCLEOSIL - the standard silica
Phase
NUCLEOSIL 100-5 C18
USP listing
L1
Surface chemistry
Medium density monomeric octadecyl (C18, ODS) modification
Mode
Reversed phase (RP)
Type
EC HPLC column (analytical)
Hardware
Stainless steel
Column length
125 mm
Column inner diameter
4 mm
Particle type
Fully porous particles (FPP)
Particle shape
Spherical
Particle size
5 µm
Eluent in HPLC column
Acetonitrile – water
Endcapped
Yes
Carbon content
15.0 %
pH stability
2.0–8.0
Pore size
100 Å
Specific surface according to BET
350 m²/g
Temperature stability (max)
60 °C
Pressure stability (max)
400 bar
Column volume
1.56 mL
DESCRIPTION
This HPLC column is packed with endcapped C18 (ODS, octadecyl silyl) modification, based on the original NUCLEOSIL silica gel. It is one of the first spherical silicas for HPLC and was originally developed by MACHEREY-NAGEL in 1974. It is exclusively manufactured in Germany and is still one of the most used HPLC silicas in quality control around the globe.