300 Å phase for proteins and peptides, Reversed phase, RP applications HPLC
Storage temperature
RT
Scope of delivery
1x analytical HPLC column, instruction leaflet, certificate of analysis
Hazardous material
No
Method
HPLC, analytical
Brand
NUCLEODUR
Base material
NUCLEODUR - high purity silica
Phase
NUCLEODUR 300-5 C18 ec
USP listing
L1
Surface chemistry
Medium density monomeric octadecyl (C18, ODS) wide-pore modification
Mode
Reversed phase (RP)
Type
EC HPLC column (analytical)
Hardware
Stainless steel
Column length
150 mm
Column inner diameter
4 mm
Particle type
Fully porous particles (FPP)
Particle shape
Spherical
Particle size
5 µm
Eluent in HPLC column
Acetonitrile – water
Endcapped
Yes
Carbon content
4.0 %
pH stability
1.0–9.0
Pore size
300 Å
Specific surface according to BET
100 m²/g
Temperature stability (max)
60 °C
Pressure stability (max)
450 bar
Column volume
1.9 mL
DESCRIPTION
Standard RP phase with wide pores (300 Å) for separation of biomolecules in analytical columns. This octadecyl modification is based on high purity NUCLEODUR silica gel.
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