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Catalog No. MN720245.40
Part No. 720245.40
Supplier MACHEREY‑NAGEL
EC HPLC column (analytical), NUCLEOSIL 300-5 C4 MPN, 5 µm, 250x4 mm
Net Price
Special offer
Availability

6 weeks

PROPERTIES
  • Recommended application(s)
    Biological macromolecules, HPLC
  • Storage temperature
    RT
  • Scope of delivery
    1x analytical HPLC column, instruction leaflet, certificate of analysis
  • Hazardous material
    No
  • Method
    HPLC, analytical
  • Brand
    NUCLEOSIL
  • Base material
    NUCLEOSIL - the standard silica
  • Phase
    NUCLEOSIL 300-5 C4 MPN
  • USP listing
    L26
  • Surface chemistry
    Special butyl (C4) modification
  • Mode
    Special application or diverse
  • Type
    EC HPLC column (analytical)
  • Hardware
    Stainless steel
  • Column length
    250 mm
  • Column inner diameter
    4 mm
  • Particle type
    Fully porous particles (FPP)
  • Particle shape
    Spherical
  • Particle size
    5 µm
  • Eluent in HPLC column
    Methanol
  • Endcapped
    No
  • Carbon content
    Not available or proprietary
  • pH stability
    1.0–9.0
  • Pore size
    300 Å
  • Specific surface according to BET
    100 m²/g
  • Temperature stability (max)
    60 °C
  • Pressure stability (max)
    250 bar
DESCRIPTION
Special reversed C4 (butyl) phase with monomerically bonded alkyl chains, brush type structure. Based on the original NUCLEOSIL silica gel. It is one of the first spherical silicas for HPLC and was originally developed by MACHEREY-NAGEL in 1974. It is exclusively manufactured in Germany and is still one of the most used HPLC silicas in quality control around the globe. Predominantly hydrophobic forces with a small portion of hydrophilic interactions. The dynamic protein binding capacity per g packing - 14 mg BSA, 27 mg cytochrome C. especially suited for the purification of larger, hydrophobic peptides and very different proteins. Maximum separation efficiency can be achieved when the injected protein mass does not exceed 1–2 % of the maximum protein loading capacity.
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