DESCRIPTION
The Human T Cell Mediated Killing Kit was designed for ease of use in multiplexing cell phenotype and function markers along with bead-based, secreted protein profile measurements in the same assay. This one wash assay requires minimal hands on time to provide the following biological insights:
Live immune cells are distinguished from dead cells by staining with a fluorescent membrane integrity dye.
Cells are immunophenotyped by staining with a fluorescent antibody panel to separate CD3+ T cells, CD3- non-T cells, CD8+ T cytotoxic cells, and CD3+ CD8- T helper cells.
The panel also includes 2 different T cell functional markers, CD25 and PD-1.
Optimized workflow enables the measurement of pro-inflammatory effector secreted cytokine IFNγ and pro-apoptotic serine protease Granzyme B in the same assay well.
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