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Catalog No. CYT17014905
Part No. 17014905
Supplier Cytiva
Sepharose 4 Fast Flow
Net Price
Special offer
Availability

4 weeks

PROPERTIES
  • Pack size
    1 L
  • Chemical stability
    Stable in commonly used aqueous buffers, 2.0 M NaOH, 70% ethanol, 30% isopropanol, 30% acetonitrile, 1% SDS, 2% Asepto, 6 M guanidine-hydrochloride, 8 M urea
  • Storage
    4 to 30°C, 20% Ethanol
  • Certificate of Analysis
    Yes
  • BioProcess resin
    Yes
  • Matrix
    cross-linked, 4% agarose
  • pH stability, CIP
    2–14
  • pH stability, operational
    3–13
DESCRIPTION
Sepharose 4 Fast Flow is a size exclusion chromatography resin developed for industrial processing at high flow rates and moderate pressures. Industrial-scale separation of large molecules and virus particles. High flow velocities at moderate pressures deliver good resolution with speed Low non-specific interactions and excellent recoveries Sepharose 4 Fast Flow size exclusion chromatography resin is based on cross-linked 4% agarose matrix, which gives good physical stability and chromatographic qualities. The matrix was developed specifically to meet the high throughput demands of industrial process separations. The rigidity permits high flow rates, which in turn gives good resolution in a minimum of time. Sepharose Fast Flow matrices enable the rapid processing of large volumes making these resins an excellent choice for use at process-scale. Sepharose Fast Flow size exclusion chromatography resins are easy to work with, and tolerate working conditions of temperature, pH and chemical agents, including cleaning-in-place (CIP) reagents, typically used in biopharmaceutical production processes.Two different Sepharose Fast Flow resins are available, Sepharose 4 Fast Flow (Fractionation range for globular proteins 4×10⁴ – 3×10⁷) and Sepharose 6 Fast Flow (Fractionation range for globular proteins 1×10⁴ – 4×10⁶)As a BioProcess resin, Sepharose 4 Fast Flow, meets industrial demands with security of supply and comprehensive technical and regulatory support.
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