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Catalog No. CYT17017001
Part No. 17017001
Supplier Cytiva
DEAE Sephadex A-25, 100 g
Net Price
412
Availability

4 weeks

PROPERTIES
  • Chromatography technique
    Ion Exchange Chromatography
  • Pack size
    100 g
  • Autoclavable
    In 0.1 M sodium chloride at 121°C for 30 min.
  • Chemical stability
    Stable to commonly used aqueous buffers additives such as 8 M urea and 6 M guanidine hydrochloride.
  • Storage
    4 to 30°C, dry resins. Used resins 4 to 8 °C in 20% Ethanol or 0.01 M NaOH
  • BioProcess resin
    No
  • Ion Exchanger Type
    Weak and strong anion
  • Ionic Capacity
    3-4 mmol/g
  • Ligand
    Diethylaminoethyl
  • Matrix
    Cross-linked dextran
  • pH stability, CIP
    2–13
  • pH stability, operational
    2–9
  • Avoid Using
    Oxidising agents and dextranase
  • Binding Capacity/ml Chromatography Medium
    thyroglobulin (Mr 669 000)- 1 mg/ml drained resin), HSA (Mr 68 000)- 30 mg/ml drained resin), α-lactalbumin (Mr 14 300)- 140 mg/ml drained resin)
DESCRIPTION
DEAE Sephadex A-25 is a weak anion exchanger based on the well documented and well proven Sephadex base matrix. Weak anion exchanger suitable batch techniques. High binding capacities. Sephadex ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix. These groups are attached to glucose units in the matrix by stable ether linkages. Different types of ion exchangers (DEAE, QAE and CM) and two different porosities (A-25/C-25 and A-50/C-50) are available.
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