CYT17128701 - Cytiva - Resins - Chromatography - Lab supplies

: Catalog No. CYT17128701

Part No. 17128701
Supplier Cytiva

ANX Sepharose 4 Fast Flow (High Sub), 500 mL

Net Price

1107

Availability

4 weeks

PROPERTIES

Pack size

500 mL
Chemical stability

Stable in commonly used aqueous buffers: 1.0 M NaOH, 20% and 70% ethanol, 8 M urea, 8 M guanidine HCl, 1M acetic acid, aqueous buffers -1.0 M NaOH, - 20 and 70% Ethanol
Storage

4 to 30°C, 20% Ethanol
BioProcess resin

Yes
Ion Exchanger Type

Weak anion exchanger
Ionic Capacity

0.13-0.18 mmol Cl^-/ml medium
Ligand

Diethylaminopropyl
Matrix

cross-linked agarose, 4%, spherical
pH stability, CIP

2–14
pH stability, operational

3–13
Ligand Coupling Method

Ether linkages and hydrophilic spacer arm

DESCRIPTION

ANX Sepharose 4 Fast Flow (high sub) is a weak anion exchanger, with a different selectivity compared with traditional ion exchangers. It is optimized for the separation of larger proteins Weak anion exchanger with different selectivity compared with traditional ion exchangers. Applicable for separation of high molecular weight proteins. Developed in co-operation with leading large-scale pharmaceutical manufacturers. High chemical stability allows for well proven CIP and sanitization protocols. The hydrophilic nature of the base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool. ANX Sepharose Fast Flow (high sub) is part of the Sepharose Fast Flow ion exchange platform, which has been the industrial standard for ion exchange chromatography during recent decades. ANX Sepharose 4 Fast Flow (high sub) is a weak anion exchanger resin with tertiary amine groups attached to the base matrix Sepharose 4 Fast Flow via ether linkage and a hydrophilic spacer arm. The diethylaminopropylgroup is coupled in a mode that precludes formation of quaternary groups, which are typically seen on traditional DEAE media, resulting in a truly weak anion exchanger. The base matrix, Sepharose 4 Fast Flow, is a highly cross-linked, 4% agarose derivative with high chemical and physical stability and broad separation range. ANX Sepharose 4 Fast Flow (high sub) has high chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols. As member of the BioProcess resin range, ANX Sepharose 4 Fast Flow (high sub) meets industrial demands with security of supply and comprehensive technical and regulatory support.