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Catalog No. CYT28903134
Part No. 28903134
Supplier Cytiva
Protein G HP SpinTrap
Net Price
348
Availability

4 weeks

PROPERTIES
  • For use with
    Standard microcentrifuge.
  • Order Information
    Instruction supplied with the column.
  • Wetted Part
    No
  • Application
    Designed for small-scale sample preparation for single use: Enrichment of target protein \nProtein-protein interaction studies \nUpstream of gel electrophoresis, liquid chromatography and mass spectrometry
  • Binding Capacity/Column
    > 1 mg human IgG/column
  • Sample volume
    600 μl
  • Storage
    2 to 8°C, 20% Ethanol
DESCRIPTION
Protein G HP SpinTrap is a prepacked, single-use spin column containing Protein G Sepharose High Performance. For simple, small-scale purification of antibodies or enrichment of specific proteins in prepacked spin columns that are ready to use. Classic and cross-link protocols provide flexibility. Elution conditions formatted for both electrophoresis and LC-MS analysis workflows. Fast binding kinetics and high capacity provides high yield. Prepacked with Protein G Sepharose High Performance for coupling of antibodies of IgG subclasses. Easy scale-up with HiTrap Protein G HP prepacked columns. Protein G Sepharose HP has high affinity for the Fc region of IgG from a variety of species. The two options in the protein enrichment protocol allow the attached antigen to be eluted separately (cross-link protocol) or together with the antibody (classic protocol). There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.For larger scale please use Protein G HP MultiTrap and HiTrap Protein G HP
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