DESCRIPTION
In recent years, reversed-phase chromatography (RPC) has become a widely accepted tool for the separation of proteins, peptides, and other biomolecules. For many applications, Waters Symmetry®, Symmetry300™, and Atlantis™, and XTerra® chemistries can be successfully used for the isolation and analyses of these biocompounds. However for some applications, the large pore size and high chemical stability of Waters BioSuite™ pC18 and pPhenyl resin-based packings may be preferred. Waters BioSuite™ RPC column offerings include a C18 (pC18) and a phenyl (pPhenyl) chemistry bonded to a pH stable, methacrylic ester-based polymeric resin. The 500 angstrom pore size of the pC18 base matrix accommodates proteins up to 2,500,000 Daltons while the 1,000 angstrom pore size of the pPhenyl base matrix accommodates proteins up to 5,000,000 Daltons. The BioSuite™ pC18, 2.5 µm, NP column contains a nonporous chemistry that yields superior chromatographic resolution compared to chromatography performed on the porous, pC18, 500, 7 µm RPC selection. However, greater protein or peptide binding capacity is possible using the porous, pC18, 500, 7 µm RPC column. The pC18 and pPhenyl RPC chemistries are available in 21.5 x 150 mm columns for “lab scale” isolations while a 2.0 mm x 75 mm column is well suited for narrow-bore HPLC and /or LC/MS applications.
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