AcroPrep™ 24-well filter plates with Omega™ membrane, a Pall Life Sciences product, can be used for ultrafiltration applications including desalting, diafiltration, concentration or fractionation of protein and nucleic acid samples. The Omega ultrafiltration membrane is a polyethersulfone (PES) membrane specifically modified to minimize protein and nucleic acid binding. The Omega membrane works on the premise of size exclusion, where samples are fractionated by molecular weight. Larger molecules can be separated from smaller molecules by selecting the appropriate molecular weight cut-off (MWCO) and filtering the sample through these plates by either centrifugation, vacuum, or positive pressure. Typical results are ≥ 90% recovery of target molecules. The 24-well filter plates are available with a variety of MWCOs, ranging from 1 kDa to 100 Kda, to use with a wide range of biomolecules. Receiver plates are supplied with the filter plates to ensure the easy collection of the filtrate.Applications Concentration and purification of peptides, proteins, oligonucleotides, DNA, and RNA Clean-up of labelling and PCR reactions Desalting and buffer exchange Fractionation based on size exclusion AcroPrep 24-Well Filter Plate Features Receiver plate and lid are included Intrinsic plate and membrane properties minimize sample loss from non-specific binding Automation friendly – compatible with all major automation platforms Designed in accordance with the ANSI/SLAS X-2004 standards Vacuum compatible – Compatible with all popular vacuum and positive pressure manifolds Centrifugation – Suitable for centrifugation with compatible rotors Specifications Filter Media Omega (modified polyethersulfone membrane) Plate Housing Polypropylene Lid Polystyrene Dimensions Length: 12.8 cm (5.0 in.) Width: 8.6 cm (3.4 in.) Height (With receiver plate): 73.9 mm (2.9 in.) Height (Without receiver plate): 37.4 mm (1.5 in.) Well-Bottom Area: 1.6 cm2 (0.24 in.2) Recommended Working Volume 7 mL for vacuum 6 mL for centrifugation Recommended Operating Vacuum 38 cm Hg (15 in. Hg) Recommended Centrifugal Force 1,500 x g Recommended Positive Pressure 50 psi