AcroPak™ 20 filters with Supor™ EKV membrane, a Pall Life Sciences product, feature built-in MachV asymmetric prefilter layer for maximum flow and throughput performance. Newer technology recommended for simplified scale-up Disposable design reduces labor costs associated with assembling, cleaning, and testing stainless steel filter holders Encapsulated format for ease of use Extended life with built-in MachV asymmetric prefilter layer for maximum flow and throughput performance Low adsorption and high chemical compatibility with hydrophilic polyethersulfone membrane for high compatibility over the entire pH range and low protein binding Gamma-irradiated, non-pyrogenic, and non-cytotoxic Inherently hydrophilic for reliable integrity testing Materials of Construction Filter Media: Supor EKV [hydrophilic polyethersulfone (PES)] membrane Housing, Vent Plug, and Support Material: Polypropylene Filling Bell: Polycarbonate (no filling bell on sanitary flange option) Sealing Technology: Thermal bonding without adhesives Pore Size Effective Filtration Area 20 cm2 Housing, Vent Plug, and Support Material: Polypropylene Filling Bell: Polycarbonate Sealing Technology: Thermal bonding without adhesives Pore Size 0.2 μm Effective Filtration Area 20 cm2 Dimensions Housing Length: 8.3 cm (3.3 in.) Housing Diameter: 6.7 cm (2.7 in.) Inlet/Outlet Connections Stepped hose barbs 6.4 - 12.7 mm (1/4 - 1/2 in.) diameter with female slip luer ID in the hose barb Typical Hold-Up Volume (with 10 psi air purge) Maximum Operating Temperature 60°C (140°F) at 1.0 bar (100 kPa, 15 psi) Maximum Operating PressureDimensions Housing Length: 8.3 cm (3.3 in.) Housing Diameter: 6.7 cm (2.7 in.) Inlet/Outlet Connections Stepped hose barbs 6.4 - 12.7 mm (1/4 - 1/2 in.) diameter with female slip luer ID in the hose barb Typical Hold-Up Volume (with 10 psi air purge) Maximum Operating Temperature 60°C (140°F) at 1.0 bar (100 kPa, 15 psi) Maximum Operating Pressure 4.1 bar (410 kPa, 60 psi) at 21 - 24°C (70 - 75°F) Recommended Integrity Test Minimum Bubble Point - Water 3.32 bar (332 kPa, 48 psi) Bacterial Retention Lot samples retain a minimum of 107 cfu/cm2 of B. diminuta per modified ASTM F838, current revision Endotoxin Level Biological Safety Passes United States Pharmacopeia (USP) Biological Reactivity Test, In Vivo Sterilization Can withstand one autoclave cycle at 125°C (257°F) for 60 minutes; water wet capsule prior to autoclaving PN 12247 is sterilized by gamma irradiation and individually packaged Applications Ideal for filtering aqueous solutions, cell culture media, and serum Small- to medium-volume sterile filtration, typically up to 2 L Suitable for use with fluids containing dilute proteins, preservatives, or other critical components Sterile filtration of media and buffers